Cell-free DNA (cfDNA) extraction represents a critical first step in liquid biopsy applications, with methodological choices significantly impacting downstream analytical results. This protocol-focused review examines established techniques for isolating cfDNA from biological fluids, with particular attention to pre-analytical variables, extraction efficiency, and quality control meas... https://www.cd-genomics.com/epigenetics/resource-cfdna-extract-materials-protocol-case.html